WebThe Ambion NorthernMax-Gly Kit is an alternative to formaldehyde-based systems for northern analysis and uses a glyoxal/DMSO Loading Solution for sample denaturation. … WebNorthernMax® Kits P/N AM1940, AM1946 Ambion NorthernMax Kits: NorthernMax, and NorthernMax-Gly, combine ultrasensitive, reliable Northern blot protocols with unsurpassed quality con-trol to ensure optimal results in less time. …

Northern Blotting Thermo Fisher Scientific - JP

WebThe DIG Northern Starter Kit produces DIG-labeled RNA probes that can be used in conjunction with the supplied chemiluminescent detection reagents for northern blotting … WebAbsolute quantitation measures the absolute amount (e.g. 5.3 x 105 copies) of a specific mRNA in an experimental sample. Dilutions of a synthetic sense strand RNA (identical or very similar to the target template) are co-amplified or detected along with the endogenous target. The sense RNA creates a concentration curve to which the endogenously … chkd emergency medicine physicians

Thermo Fisher Scientific - US

Web2 de dez. de 2011 · Hybridization conditions and buffers were according to NorthernMax kit (Ambion) manufacturer instructions. RESULTS. To obtain transcriptome data for S. solfataricus P2, we sequenced a total RNA sample from this archaeon grown on organotrophic medium to a stationary phase (Methods). WebNorthernMax Kit contains a complete set of RNase-free reagents for running formaldehyde-based northern analysis. Sufficient reagents are provided to process 1000 cm. 2. membrane. Key features of the kit include: • Increased sensitivity up to 100x over standard hybridization protocols. • Reduced hybridization time (2 hours) for many messages. chkd education