Fitc staining protocol
WebApplication Notes. Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections 5 and formalin-fixed paraffin-embedded tissue sections. WebTransfer 100 µL of cell suspension in a 5 mL test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 5 µL of 7-AAD Viability Staining Solution. 5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark. 6. Add …
Fitc staining protocol
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WebJan 1, 2024 · MitoTracker green and mitotracker red staining for functionality assay. ... FITC – Green Excitation⁄Emission (nm): 490/516 ... This protocol contains a compilation … WebDec 12, 2024 · All proteins were analysed on 15% SDS-PAGE followed by coomassie staining. (D) Dialysed FITC labelled Pea15 elute. ... In the present study, we have modified the conventional FITC labelling protocol by introducing the TAP tags at the N- and C- termini of the target protein. We have also modified the commercially available pMALc5E …
WebFixable Dyes - Dead cells allow fixable viability dyes to cross their membranes where they stain intracellular amines that are more abundant in the cytoplasm than the extracellular amines on the surface of live cells. Cells can be formaldehyde fixed post staining. Cells stained with these products can also be run unfixed. WebMar 17, 2024 · Us discuss FITC stamping protocols, how FITC verb mill, FITC labels and stains, and applications of FITC in literature. Toggle ship. Home; Browse Conjugation Kits; Contact Us; ... Product Description Mix-n-Stain™ FITC Antibody Labeling Kits allow i for label ≤5 ug up to 100 ug of your drug with fluorescein (FITC) in just 30 minutes, with ...
WebFITC anti-human CD55 Antibody - CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). ... For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. ... Cell Surface ... WebAcrosome membrane integrity was evaluated following the protocol of ref. 54 , in which sperm were stained with PNA-FITC (final concentration: 1.2 µM) for 5 min at 38°C in the …
WebCD98 is composed of a light chain (45 kD) and a heavy chain (80 kD). Studies with knock-out mice indicate that the CD98 heavy chain is involved in integrin-dependent cell spreading and protection against anchorage deprivation-induced apoptosis. The MEM-108 antibody is useful for flow cytometry and immunoprecipitation.
http://drmr.com/abcon/FITC.html litchfield county courthsoueWebFluorescence Microscopy Analysis of Annexin V-FITC Staining. HeLa cells were left untreated [top left panel] or induced to undergo apoptosis with camptothecin (8µM camptothecin, 24 h) [top middle panel]; or staurosporine (1µM, 4 h) [top right panel] and stained with Annexin V-FITC according to the protocol provided. bottom left panel, … litchfield county ct election resultsWeb5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HA2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested). Protocol litchfield county ct assessorWebReagents and Protocol (adapted from Invitrogen): ... V-FITC and an antibody conjugated to APC. Because APC is excited by the Red (637 nm) laser, FITC is excited by the Blue (488 nm) laser, and PI ... Annexin V staining becomes detectable and then, as the cells become permeable, PI gains access to the DNA. References: litchfield county connecticut zillowWebProtocols of Sigma and MPBio are the same. They suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in DMSO) per 1 ml of protein solution. Final molar F/P ... litchfield county couthsoueWebMar 19, 2024 · Throughout the whole staining procedure, tissue sections need to be protected from direct light exposure as fluorescently (e.g., FITC/TRITC-) labeled lectins quench more quickly than Alexa Fluor-labeled probes. Additionally, stained sections should be imaged within 4 days to ensure specific binding. litchfield county home assistanceWebAvidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.125 µg per 10 6 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application. litchfield county ct history